Session 6 - Joint Session: Pharma and Biopharma Applications
Session Chair
Dr Anne-Catherine SERVAIS
UNIVERSITY OF LIÈGE, Liege, Belgium
Anne-Catherine Servais obtained her PhD degree in Pharmaceutical Sciences at the University of Liege on chiral separation in nonaqueous capillary electrophoresis in 2005. During her postdoctoral research, she joined in 2008 the group of Professor de Jong (Department of Biomedical Analysis, Utrecht University) to study the coupling of nonaqueous capillary electrophoresis to mass spectrometry. Currently, she is Associate Professor at the Laboratory for the Analysis of Medicines (University of Liege) directed by Professor Fillet. She performs research in the development of innovative separation methods using miniaturized techniques for the analysis of biomarkers (metabolites, peptides and proteins), biopharmaceuticals including biological entities (virus-like particles) but also for the study of their molecular interactions. She is a (co)author of more than 80 peer-reviewed papers, with an H-index (Scopus) of 26.
09:00
KL07 - Characterization of Lipid Nanoparticles for mRNA Vaccines by Taylor Dispersion Analysis and Capillary Electrophoresis
Prof. Hervé COTTET
UNIVERSITY OF MONTPELLIER, Montpellier, France
Hervé Cottet (48) is full professor at the Max Mousseron Biomolecules Institute (IBMM) in Montpellier (France). Since 2012, he is the head of a Research Department (~15 permanent researchers) at the IBMM. His research work concentrates at the interface between separation sciences, pharmaceutical sciences, physical chemistry and polymers. He is developing CE methodologies and Taylor Dispersion Analysis (TDA) for the characterization of biopharmaceuticals (including vaccines), (bio)polymers, polyelectrolytes, drug delivery systems, dendrimers, nanoparticles, colloids and bacteria. He is both interested in fundamentals (mobility modeling, electrophoretic behavior) and practical (or industrial) applications of CE and TDA. Graduated from the Ecole Supérieure de Physique et Chimie Industrielles de Paris (ESPCI), he completed his PhD in analytical chemistry in 1999 at the Ecole Nationale Supérieure de Chimie de Paris (ENSCP- PSL, France) on Capillary Electrophoresis (CE). After a post-doc at the Technical University of Eindhoven (the Netherlands), he joined the University of Montpellier in 2000. He has co-authored more than 130 scientific articles dealing with CE and/or TDA.
09:30
OC15 - Exploiting Diverse Glycosylation Strategies for Optimized Glycoconjugated Vaccines
Dr Sara TENGATTINI
UNIVERSITY OF PAVIA, Pavia, Italy
Sara Tengattini graduated in Pharmaceutical Chemistry and Technology at the University of Pavia, Italy. Then, she obtained her PhD discussing a thesis entailed “Development of innovative analytical methods for the characterization of potential neo-glycovaccines against tuberculosis”. Currently she is a researcher at the Department of Drug Sciences of the University of Pavia. Her research activities are mainly related to the development of analytical methodologies for the characterization of therapeutic proteins, with particular regards to monoclonal antibodies and glycoconjugate vaccines.
09:50
OC16- Chip-Sized Cell Gene Modification System for Integration into Modular Microfluidics
Dr Hashim ALHMOUD
BILKENT UNIVERSITY, Ankara, Turkey
Hashim Alhmoud is an early career researcher and an EC Marie-Curie Actions Fellow at Bilkent University's Department of Mechancial Engineering, Turkey. In 2016, Hashim obtained his PhD from the University of South Australia where he developed novel nanoparticle drug delivery systems for cell therapy and tissue engineering. He took up his first post-doctoral appointment between the years of 2017 and 2020 at Monash University's Faculty of Pharmacy in Melbourne Australia, where he continued his work on nanoengineered interfaces for cell modification and nanoscale delivery vectors. While there, he published over 10 peer-reviewed journal articles and participated in several industry-driven projects. He later joined Bilkent University and the National Nanotechnology Research Center in Turkey as a Marie-Curie Actions Fellow in 2021. His current research focuses on developing effective microfluidic modules for cell probing and modification with single-cell precision.
10:10
OC17 - Absolute Quantification and Structural Characterization of Infliximab in Human Serum Using Capillary Electrophoresis Hyphenated to Tandem Mass Spectrometry
(YS)
Ms Tessa REINERT
UNIVERSITY OF STRASBOURG, Strasbourg, France
Tessa Reinert graduated in analytical chemistry at the University of Strasbourg in 2020. She is currently a 2nd year PhD student in co-direction between the Laboratory of Mass Spectrometry of Interactions and Systems (LSMIS) in Strasbourg and the Chemical and Biological Technologies for Health Unit (UTCBS) in Paris. Her PhD mainly focuses on the development of an analytical method for monoclonal antibody characterization and quantification.
10:30
Coffee break & Exhibition
11:00
OC18 - From Intact to Bottom-Up: Multidimensional Platform for Comprehensive Characterization of Monoclonal Antibodies in Crude Broths and Formulations from a Single Injection
(YS)
Mrs Arta SADIGHI
VRIJE UNIVERSITEIT AMSTERDAM , Amsterdam, The Netherlands
Im doing my Ph.D. in the Netherlands in the group of Govert Somsen and Anouk Rijs where I focus on the hyphenation of different analytical techniques for the characterization of proteins. These include monoclonal antibodies (mAbs) and proteins that cause neurological diseases. Different online platforms are created to provide in-depth information on structural heterogeneity, post-translational modifications, and aggregation that may affect the activity, function, and stability of proteins.
11:20
OC19 -In-Vitro Drug-Induced Liver Injury Monitoring Using Raman Spectroscopy
(YS)
Ms Margot VANDERMOTTEN
VRIJE UNIVERSITEIT BRUSSEL, Elsene, Belgium
Margot Vandermotten is a PhD student at Brussels Photonics, VUB. She obtained her Bachelor’s degree in Electromechanical Engineering in 2019 and her Master’s degree in Biomedical Engineering in 2021 both from the Vrije Universiteit Brussel (VUB). Her research currently focuses on the use of optical detection techniques to identify the presence of Drug-Induced Liver Injuries (DILI) in liver in-vitro models
11:40
OC20 - All-Integrated Droplet Microfluidics Station for High-Throughput Single Cell Screening and Sorting
Dr Stéphanie VAN LOO
LIVEDROP, Liege, Belgium
Graduated biomedical engineer from University of Liège in 2010, Stéphanie van Loo did a PhD thesis in the Microfluidics lab of the Aerospace and Mechanical Engineering department of the University of Liège from 2013 to 2017, entitled Droplet microfluidic for single-cell manipulation, under the supervision of Prof Tristan Gilet. During her PhD, she set up an entire droplet microfluidics set-up from scratch, starting from the design and fabrication of microfluidic chips. She collaborated with several biologists.
After her PhD, she wanted to make this fascinating technology available to all biologists, so she obtained funding from the Walloon Region to create a spin-off company whose objective would be to develop and commercialize droplet microfluidics instruments for biologists. She also worked as post-doc in the Unit of Animal Genomics of the GIGA research center of the University of Liège, during which she applied her technology to fundamental genomics research area.
She created the company LiveDrop in April 2022 to market the ModaFlow instrument.
Session Chair
Prof. Jeroen LAMMERTYN
KULEUVEN, Leuven, Belgium
Jeroen Lammertyn is Full Professor at the Faculty of Bioscience engineering and head of MeBioS Division of the Biosystems Department at KU Leuven. He founded the MeBioS-Biosensors group in 2005, which currently counts 35 researchers. The lab focuses its research on the development of novel bio-molecular detection concepts and miniaturized analysis systems. He is (co)author of >250 peer reviewed research papers (h-index = 62, 14430 citations, average impact factor of 10 best publications = 15.9) and over 150 conference papers and book chapters. He was/is Technical Program Committee member of the 3 most important and largest conferences in the field of lab-on-a-chip technology (MEMS, MicroTAS and Transducers) and he is often invited to give lectures and keynote talks. He has established many research collaborations at the national and international level (e.g., 8 EU-projects). This has resulted in an accumulated project funding of over 30 M€ (>35 (inter)national projects funded by KUL, FWO, IWT, VLK, H2020, Bill and Melinda Gates Foundation, etc). Jeroen is co-inventor of 12 patent applications and founder of a spin-off company on fiber-optic biosensor (FOx Biosystems). During his career, he was a laureate of >10 awards (e.g. Quetelet Prize in Biostatistics, BiR&D Award 2014 for most original multidisciplinary PhD with high industrial valorization potential, …). Jeroen Lammertyn is involved in teaching 7 courses in the B.Sc. and M.Sc. programs of Bioscience Engineering and the Master in Nanoscience and Nanotechnology at the KU Leuven.
12:00
12:30
13:00
Lunch Seminar - EU-funded ACTPHAST4 Researcher project
Prof. Francis BERGHMANS
VUB, Brussels, Belgium
13:30
14:30
Session Chair
Prof. Govert SOMSEN
VU UNIVERSITY AMSTERDAM, Amsterdam, The Netherlands
Govert Somsen is full professor of Biomolecular Analysis/Analytical Chemistry at the Vrije Universiteit Amsterdam, The Netherlands. He obtained his doctorate in Amsterdam, and subsequently was assistant and associate professor at the University of Groningen and Utrecht University in The Netherlands. His main expertise is on hyphenated concepts in separation science directed to the characterization of (bio)polymers and (bio)active compounds. His group made significant contributions to the development, optimization and application of coupled analytical techniques combining selective liquid-phase separations with mass spectrometry, optical spectroscopy and bioactivity assays. He is (co-)author of over 200 papers in these fields, and editor of Journal of Chromatography B.
14:30
KL08 - Native CZE-MS of Proteins and Protein Complexes
Dr Kevin JOOSS
NORTHWESTERN UNIVERSITY, Evanston, United States
Kevin Jooß obtained his B.Sc. in Chemistry and M.Sc. in Analytical and Bioanalytical Chemistry from Aalen University in 2013 and 2014, respectively. He joined the research group of Prof. Christian Neusüß (Aalen University, Germany) and earned his Ph.D. in collaboration with the Technical University of Munich in 2019 developing multidimensional electromigrative separation approaches coupled to mass spectrometry. Afterwards, Dr. Jooß moved to Northwestern University (Evanston, IL, USA) as a postdoctoral fellow joining the lab of Prof. Neil Kelleher driving capillary electrophoresis-centric techniques in the context of denatured and native top-down proteomics. His interests include, amongst others, exploring the characteristics of protein separation under native conditions, nucleosome characterization in the context of epigenetic research, and promoting adaption of CE for top-down applications.
15:00
OC21 - Imaged Capillary Isoelectric Focusing Combined with MS And Bioactivity Detection for Characterisation of Snake Venoms
(YS)
Ing Mari-Anne ASSELER
VRIJE UNIVERSITEIT AMSTERDAM, Amsterdam, The Netherlands
Mari-Anne Elles Asseler is from the Netherlands. Even though the Netherlands only has three native snake species, of which one is venomous. She has had the opportunity to research several venomous snake species from around the world. Her research involves looking at the native protein structures, using imaged capillary isoelectric focusing (iCIEF) in combination with bioactivity assays and MS for the analysis of crude snake venoms.
15:20
OC22 - Optimizing Intact Protein Separation Efficiency Using Capillary Electrophoresis
(YS)
Ms Laura DHELLEMMES
UNIVERSITY OF MONTPELLIER, Montpellier, France
Laura Dhellemmes received her Master of Engineering degree from ENSIACET, Toulouse in 2020. She is currently investigating SMIL coatings used in CE as a PhD student at IBMM, Montpellier, France, in the context of a ANR-PRCI project in collaboration with Prof. Christian Neusüß and Prof. Norbert Schaschke from the University of Aalen, Germany.
Session Chair
Prof. Tristan GILET
UNIVERSITY OF LIÈGE, Liège, Belgium
14:30
KL09 - Novel Microfluidic Platform for B-cell Screening and Retrieval: A Case Study on Anti-SARS-CoV-2 Monoclonal Antibody Discovery
Prof. Jeroen LAMMERTYN
KULEUVEN, Leuven, Belgium
Jeroen Lammertyn is Full Professor at the Faculty of Bioscience engineering and head of MeBioS Division of the Biosystems Department at KU Leuven. He founded the MeBioS-Biosensors group in 2005, which currently counts 35 researchers. The lab focuses its research on the development of novel bio-molecular detection concepts and miniaturized analysis systems. He is (co)author of >250 peer reviewed research papers (h-index = 62, 14430 citations, average impact factor of 10 best publications = 15.9) and over 150 conference papers and book chapters. He was/is Technical Program Committee member of the 3 most important and largest conferences in the field of lab-on-a-chip technology (MEMS, MicroTAS and Transducers) and he is often invited to give lectures and keynote talks. He has established many research collaborations at the national and international level (e.g., 8 EU-projects). This has resulted in an accumulated project funding of over 30 M€ (>35 (inter)national projects funded by KUL, FWO, IWT, VLK, H2020, Bill and Melinda Gates Foundation, etc). Jeroen is co-inventor of 12 patent applications and founder of a spin-off company on fiber-optic biosensor (FOx Biosystems). During his career, he was a laureate of >10 awards (e.g. Quetelet Prize in Biostatistics, BiR&D Award 2014 for most original multidisciplinary PhD with high industrial valorization potential, …). Jeroen Lammertyn is involved in teaching 7 courses in the B.Sc. and M.Sc. programs of Bioscience Engineering and the Master in Nanoscience and Nanotechnology at the KU Leuven.
15:00
OC23 - Highly Accurate and Flexible Serial Picoinjection in Droplet Microfluidics
(YS)
Ing Jolien BREUKERS
KU LEUVEN, Heverlee, Belgium
Jolien Breukers received a Master of Science degree in Bioscience Engineering at KU Leuven, Belgium, in 2017. Afterwards, she joined the Biosensors group at the Biosystems department of KU Leuven to pursue a PhD. In her research, she is developing novel microfluidic technologies for single-cell analysis, for which she mostly focuses on droplet microfluidics, continuous microfluidics and microwell array technologies.
15:20
OC24 - Microfluidic Droplet Instrumental and Methodological Development for Glycan Sample Processing Towards CDG Diagnostic Application
(YS)
Mr Théo LIÉNARD-MAYOR
INSTITUT GALIEN PARIS-SUD, Châtenay-Malabry, France
Théo Liénard obtained his Master’s degree in fundamental physics from Université Paris-Saclay in 2018, with a specialization in microfluidics from Institut Pierre Gilles de Gennes. He is currently a 3rd year PhD student at Institut Galien Paris-Saclay in Myriam Taverna’s team, working on microfluidic solutions for both sample treatment and analysis of glycans by capillary electrophoresis with laser induced fluorescence detection (CE-LiF) for diagnosis of congenital disorders of glycosylation (CDGs).
Prize session - Sciex Innovation Award
Session Chair
Prof. Vincent REMCHO
OREGON STATE UNIVERSITY, Corvallis, OR, 97331, United States
15:45
BA01 - Maturation of Microfluidics in an Era Where Real-world Problems Demand a Solution
Prof. James LANDERS
UNIVERSITY OF VIRGINIA, VA, United States
James Landers is a Jefferson Scholars Faculty Fellow and Commonwealth Professor of Chemistry, Professor of Mechanical Engineering, and an Associate Professor of Pathology at the University of Virginia. After obtaining degrees in Biochemistry from the University of Guelph (Canada), he did postdoctoral fellowships in Toxicology at the Hospital for Sick Children, in Endocrine Dysfunction at the University of Toronto School of Medicine and, in Steroid Hormone Cancers as a Canadian Medical Research Council Fellow at the Mayo Clinic. He created the Clinical Capillary Electrophoresis Facility in Laboratory Medicine at Mayo Clinic, where his work on microfluidic systems began, launched his academic career at the University of Pittsburgh, and then ramped up his program at the University of Virginia (UVA) where the sole focus was on developing microscale analytical technology. His UVA research team developed and demonstrated the first fully-integrated microfluidic system for sample-to-result genetic analysis, and this led to efforts with his start-up collaboration with Lockheed Martin developing ‘Rapid DNA’ systems for swab-to-STR profiling, and with the National Institutes of Health developing a 25-min point-of-need qPCR test for SARS-CoV-2. He has published more than 265 papers and serves as the CoEditor-in-Chief for the journal Analytica Chimica Acta.
17:00
Walking City Tour (1 hour)
19:30